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Primer3 - PCR primer design tool

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Design PCR primers from DNA sequence. Widely used (190k Google hits for "primer3"). From mispriming libraries to sequence quality data to the generation of internal oligos, primer3 does it. C&perl. Developers/testers/documenters needed.

You can download file releases of Primer3 - PCR primer design tool project from List of release files

System Requirements

Operating System: OS Portable (Source code to work with many OS platforms), POSIX (Linux,BSD,Solaris etc..), Windows XP

DL	primer3-2.3.5.tar.gz Date: 2013-01-07, Size: 1655871

DL	primer3-2.3.5.tar.gz Date: 2013-01-07, Size: 1655871

DL	primer3-2.3.5.tar.gz Date: 2013-01-07, Size: 1655871

DL	primer3-2.3.5.tar.gz Date: 2013-01-07, Size: 1655871

DL	primer3-2.3.5.tar.gz Date: 2013-01-07, Size: 1655871

List of release files

File/Folder Name	File Type	Size	Date	Download Count
Latest 5 files
primer3web-4.0.0.tar.gz	application/x-gzip; charset=binary	374.1 KB	2013-01-24 07:27	24
primer3_manual.htm	text/html; charset=utf-8	194.1 KB	2013-01-07 07:21	25
release_notes.txt	text/plain; charset=us-ascii	23.4 KB	2013-01-07 07:20	2
primer3-2.3.5.tar.gz	application/x-gzip; charset=binary	1.6 MB	2013-01-07 07:20	227
release_notes.txt	text/plain; charset=us-ascii	21.4 KB	2012-05-31 16:35	7
All Files
primer3-web
4.0.0
primer3web-4.0.0.tar.gz	application/x-gzip; charset=binary	374.1 KB	2013-01-24 07:27	24
3.0.0
primer3web-3.0.0.tar.gz	application/x-gzip; charset=binary	373.9 KB	2012-03-21 03:13	27
0.3.0
primer3-web-htdocs-0.3.0.tar.gz	application/x-gzip	16.4 KB	2006-11-13 05:04	26
README-primer3-web-0.3.0.txt	text/plain; charset=us-ascii	3.3 KB	2006-11-13 05:04	18
primer3-web-cgi-bin-0.3.0.tar.gz	application/x-gzip	333.2 KB	2006-11-13 05:04	17
primer3
2.3.5
primer3_manual.htm	text/html; charset=utf-8	194.1 KB	2013-01-07 07:21	25
release_notes.txt	text/plain; charset=us-ascii	23.4 KB	2013-01-07 07:20	2
primer3-2.3.5.tar.gz	application/x-gzip; charset=binary	1.6 MB	2013-01-07 07:20	227
2.3.4
release_notes.txt	text/plain; charset=us-ascii	21.4 KB	2012-05-31 16:35	7
primer3_manual.htm	text/html; charset=utf-8	192.5 KB	2012-05-31 15:42	31
primer3-2.3.4.tar.gz	application/x-gzip; charset=binary	2.5 MB	2012-05-31 15:41	433
2.3.3
release_notes.txt	text/plain; charset=us-ascii	21.8 KB	2012-05-21 15:01	2
primer3_manual.htm	text/html; charset=utf-8	196.6 KB	2012-05-21 15:01	13
primer3-2.3.3.tar.gz	application/x-gzip; charset=binary	2.5 MB	2012-05-21 15:01	7
2.3.2
release_notes.txt	text/plain; charset=us-ascii	20.9 KB	2012-03-19 18:14	5
primer3_manual.htm	text/html; charset=utf-8	192.5 KB	2012-03-19 18:14	15
primer3-2.3.2.tar.gz	application/x-gzip; charset=binary	2.5 MB	2012-03-19 18:12	107
2.3.1
primer3_manual.htm	text/html; charset=utf-8	192.5 KB	2012-03-07 22:35	5
release_notes.txt	text/plain; charset=us-ascii	20.7 KB	2012-03-07 22:33	0
primer3-2.3.1.tar.gz	application/x-gzip; charset=binary	2.5 MB	2012-03-07 22:31	1
2.3.0
primer3_manual.htm	text/html; charset=utf-8	191.7 KB	2012-02-08 02:14	10
release_notes.txt	text/plain; charset=us-ascii	19.7 KB	2012-02-08 02:12	0
primer3-2.3.0.tar.gz	application/x-gzip; charset=binary	2.5 MB	2012-02-06 19:01	42
2.2.3
release_notes.txt	text/x-fortran; charset=us-ascii	15.4 KB	2010-09-17 11:52	17
primer3-2.2.3.tar.gz	application/x-gzip; charset=binary	2.2 MB	2010-09-17 11:50	422
2.2.2-beta
primer3-2.2.2-beta-patch-for-solaris.patch	text/x-c; charset=us-ascii	0.2 KB	2010-03-17 04:00	80
release_notes.txt	text/x-fortran; charset=us-ascii	15.3 KB	2010-03-12 03:18	7
primer3-2.2.2-beta.tar.gz	application/x-gzip; charset=binary	2.2 MB	2010-03-12 03:16	353
2.2.0-alpha
release_notes.txt	text/plain; charset=us-ascii	14.9 KB	2009-10-29 06:16	23
primer3-2.2.0-alpha.tar.gz	application/x-gzip	2.1 MB	2009-10-23 07:52	41
2.2.1-alpha
release_notes.txt	text/plain; charset=us-ascii	15.0 KB	2009-10-29 06:12	5
primer3-2.2.1-alpha.tar.gz	application/x-gzip	2.1 MB	2009-10-28 05:05	273
2.0.0-alpha
primer3-2.0.0-alpha.tar.gz	application/x-gzip	1.8 MB	2008-12-20 11:03	67
1.1.4
README-primer3-1.1.4.txt	text/plain; charset=iso-8859-1	59.1 KB	2008-05-15 19:14	58
primer3-1.1.4-WINXP.zip	application/x-zip	1.3 MB	2008-05-15 19:11	102
primer3-1.1.4-WINXP.checksum.txt	text/plain; charset=us-ascii	0.1 KB	2008-05-15 19:11	45
primer3-1.1.4-OSX.checksum.txt	text/plain; charset=us-ascii	0.1 KB	2008-05-15 18:33	42
primer3-1.1.4-OSX.tar.gz	application/x-gzip	997.8 KB	2008-05-15 18:33	20
primer3-1.1.4.tar.gz	application/x-gzip	951.8 KB	2008-04-29 20:05	23
primer3-1.1.4.checksum.txt	text/plain; charset=us-ascii	0.1 KB	2008-04-29 20:05	7
1.1.3
primer3-1.1.3.tar.gz	application/x-gzip	921.6 KB	2008-02-22 01:57	22
1.1.1
primer3-1.1.1-WINXP.zip	application/x-zip	1.3 MB	2007-04-03 16:06	31
primer3-1.1.1-WINXP.checksum.txt	text/plain; charset=us-ascii	0.1 KB	2007-04-03 16:06	8
primer3-1.1.1-OSX.checksum.txt	text/plain; charset=us-ascii	0.1 KB	2007-03-28 17:02	12
primer3-1.1.1-OSX.tar.gz	application/x-gzip	1.0 MB	2007-03-28 17:02	31
primer3-1.1.1.checksum.txt	text/plain; charset=us-ascii	0.1 KB	2007-03-28 15:48	18
primer3-1.1.1.tar.gz	application/x-gzip	942.5 KB	2007-03-28 15:48	28
README-primer3-1.1.1.txt	text/plain; charset=iso-8859-1	60.6 KB	2007-03-28 05:53	9
1.1.0-beta
primer3-1.1.0-beta-WINXP.checksum.txt	text/plain; charset=us-ascii	0.1 KB	2007-03-20 23:06	13
primer3-1.1.0-beta-WINXP.zip	application/x-zip	152.8 KB	2007-03-20 23:06	3
primer3-1.1.0-beta-OSX-univ-binary.checksum.txt	text/plain; charset=us-ascii	0.1 KB	2007-02-07 06:51	5
primer3-1.1.0-beta-OSX-univ-binary.tar.gz	application/x-gzip	1.0 MB	2007-02-07 06:51	6
primer3-1.1.0-beta.tar.gz	application/x-gzip	940.5 KB	2007-02-07 05:14	6
primer3-1.1.0-beta.checksum.txt	text/plain; charset=us-ascii	0.1 KB	2007-02-07 05:10	3
README-primer3-1.1.0-beta.txt	text/plain; charset=iso-8859-1	57.5 KB	2007-01-18 00:35	5
1.0.1
primer3_1.0.1_core_IBM_SLES9_binary.tar.gz	application/x-gzip	108.8 KB	2006-11-27 20:05	0
Makefile_IBM_POWER	image/x-3ds	4.7 KB	2006-11-27 20:04	0
README.primer3_1.0.1.txt	text/plain; charset=us-ascii	53.9 KB	2006-11-11 23:05	0
primer3_1.0.1.tar.gz	application/x-gzip	881.4 KB	2006-11-11 22:57	2
primer3plus
1.1.0
primer3plus_1.1.0_Source_only.zip	application/x-zip	398.0 KB	2007-04-04 22:00	13
primer3plus_1.1.0_WINXP.zip	application/x-zip	483.3 KB	2007-04-04 22:00	33
primer3plus_readme.txt	text/plain; charset=us-ascii	0.4 KB	2007-04-04 22:00	18
primer3plus_1.1.0_Linux.tar.gz	application/x-gzip	465.0 KB	2007-04-04 22:00	3
primer3plus_1.1.0_Source_Only.tar.gz	application/x-gzip	371.5 KB	2007-04-04 22:00	3
1.0.0
primer3plus_1.0.0_Windows.zip	application/x-zip	452.5 KB	2007-02-02 10:35	10
primer3plus_1.0.0_Linux_x86.tar.gz	application/x-gzip	1.3 MB	2007-02-02 10:35	3
primer3plus_1.0.0_Source_Only.tar.gz	application/x-gzip	368.1 KB	2007-02-02 10:35	11
primer3plus_1.0.0_Source_Only.zip	application/x-zip	393.6 KB	2007-02-02 10:35	7

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Search Keywords

primer3, primer, 3, design, windows, pcr, ダウンロード, tool, primer3web, for ...

Development Status: 4 - Beta, 5 - Production/Stable
Intended Audience: Science/Research
License: GNU General Public License v2 (GPLv2)
Operating System: OS Portable (Source code to work with many OS platforms), POSIX (Linux,BSD,Solaris etc..), Windows XP
Programming Language: C, Perl
Topic: Bio-Informatics, SourceForge.net
User Interface: Command-line, Web Environment

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